Singing for Summer

Even though it's only Memorial Day Weekend, I feel this is the beginning of summer. We helped open the family pool yesterday. The days are already longer. Hello, sunrise at 5:40am and sunset not until 7:55pm. Over 14h of daylight. I've been thinking about my Arabidopsis plants and how they are on a 16h light 8h dark schedule, beginning around 7:30am and ending around 11:30pm. This explains my work schedule. I feel if Arabidopsis is working 16 hour days making molecules for me, I should be keeping busy too.

I'm getting ready to attend a conference on plant adaptations to low oxygen environments. My research has been using the model plant Arabidopsis, one of the first 3 organisms to have its genome sequenced. It will be interesting to show my work to the other attendees at this conference, who come from a wide cross-section of scientific disciplines with the goal of addressing a specific scientific problem and who work with a wider diversity of plants (some adapted to grow in low-oxygen environments and some of agricultural importance). My friend Takeshi Fukao (http://genomics.ucr.edu/news/news-details.php?id=41) told me that the conference attendees will be mainly ecologists (who study life and interactions between lifeforms within ecosystems) and agronomists (who develop plants for specific purposes including food, fuel, feed and fiber). As I'm writing this now, I am supposed to be working on a poster summarizing my research project... later, right?

I'll be flying into Tuscany for sightseeing June 14-20, then attending the conference in Volterra June 20-25, then traveling to the rolling plains of central Germany (Thuringia) to work at a research institute specializing in the study of plant-insect communication. I hope to rent or buy a bicycle over there and see the areas better that way. I'm looking forward to being situated in a world-leading research center while I continue my doctoral studies. The Saale river, which bends along the east side of Jena, has cut high limestone cliffs into the landscape and supports nearby wine growing regions.

How do I feel right now? Well I feel loved and supported. I've just had visits with my sister Karly for adventures, my mom for Mother's Day, and my friend Cheri for her birthday. My husband and doggies are here right now with me, so I can't complain. There's so much to do and so little time, yet I have all the time I need. I feel I could do so many more experiments, but that's where skill comes in. Knowing one's priorities is priceless.

"Science and art belong to the whole world, and before them vanish the barriers of nationality." -Johann Wolfgang von Goethe

Next blog post, I swear, I will write about the integral membrane protein called human serotonin transporter (SERT) and the small molecules that bind to it.

Spring has Sprung!

Many changes take place in spring. Weather patterns change. Daylight savings time shifts our working hours to accommodate the sun. Our family is changing, but I suppose that the only thing that never changes is that things are always changing.

Biking time is my favorite time of day. The way I can productively reward myself is related to biking. Time spent on the bike. Only problem is that I worried about overtraining. So I added running into the mix. Still love biking best though.

Trying to get into daily affirmations. Trying to maintain this weight. Trying to be more positive. Trying to leave more free room in my schedule, with daily priority tasks that "must get done" and other than that priority, worrying less.

Like a bulldog chasing a cat

So it seems I've been called out as having a Type A habit. What does that mean exactly? Well it generally means I have a desire to do everything, quickly, better than anyone else ever has, and perfectly. Why? Because I can, and it's important, and I need to do it before someone else does, and because it will prove to everyone that I am a worthwhile human being. At least that's how Carol Lankton (a marriage and family therapist for 28 years) describes what's been going on inside my head. My cycling buddy says I ride like a bulldog chasing a cat. I hoped I was the cat in that simile, but unfortunately not. Can't I be a poodle instead of a bulldog?

The problem is that while Type A personality is correlated with assertiveness and sturdy work ethic, it is also commonly combined with anxiety/depression and tends to alienate friends and family. Researchers speculate that this may happen because society tends to shun and isolate competitive, hard-driving women, even as it encourages men with the same traits. That's why I say "I'm a grrrrrrl." Luckily my she-wolf companions (my doggies and running partners) don't mind being dominated. Call me a bitch if you like, but I'm still standing. And running. And biking.

Stormy Weather

It's the rainy season, and I'm gonna bitch about it. I rode to school today, on my road bike, ending up soaked and dirty. On the bright side, I wore black and I look good wet.

Life has been stormy, too. I've just attended the Southern California Users of Magnets (SCUM) meeting, which was co-hosted by my research advisor, mentor, and personal hero, Professor Cynthia Larive and my husband's former research advisor, Professor Len Mueller. It was sponsored (in part) by the company my husband interviewed with in Boston last month, Bruker. I feel like I have a million projects going on right now and it's only a matter of time before I screw something up. But it's all about perception, right? And for now it seems I am managing.

I've been developing some educational materials for a course called "Instrumental Methods," which involves advanced topics in analytical chemistry, such as; chromatographic separations, electrochemistry, and spectroscopic techniques. Analytical chemists are known for attention to detail, accuracy & precision, and the desire to know (1) What substance(s) are present in a "sample" and (2) How much (of each substance) is present in the "sample." One could make a career as an analytical chemist improving experimental designs for targeted analysis or validating new methods to enhance the richness of information from a nontargeted analysis. Often, the analytical chemist is challenged to balance performance and cost, lending somewhat of an engineering or applied type of problem-solving to our field. We can also see a direct application of our work in many diverse types of fields: environmental monitoring, medical devices, pharmaceutical R&D pipeline, quality control, drug enforcement, etc.

Speaking of which, the reason I'm developing these new educational materials [namely a problem-based laboratory exercise] is because UCR Department of Chemistry was the recipient of a Gas Chromatograph-Mass Spectrometer (GC-MS) donated kindly by the local Drug Enforcement Agency (DEA) lab. It's cool because it's already set up to detect small amounts of volatile organic molecules in a complex mixture, because it was likely used to detect banned substances and their downstream metabolites in urine, feces or blood.

The class of substances we're focusing on in this activity are monoterpenes. These are found in citrus peel (among other plant-based sources) and are useful for cleaning and degreasing (as in the case of turpentine) and/or for fragrances in perfumes and soaps. I have isolated monoterpenes from a variety of citrus fruits, including orange, lime and lemon, tangerine and mandarin so far. Each peel has offered a complex mixture of these molecules (between 5 to 20 detectable monoterpenes per variety of citrus) and the subtle but statistically significant differences between fruits is what we're interested in recording.

Since my other research advisor, Julia Bailey-Serres, has been training me to think like a plant biologist, I asked the question "But why does the plant produce these compounds?" Surely not for us to use as a degreaser or additive to our soaps and perfumes. It turns out that these molecules have exhibited antifungal activity in addition to deterring insects from the area. This protects the fruit from pathogen attack and allows the citrus to live long and prosper.

I found limonene in high abundance in each peel extract, while the relative amounts of the minor components in each extract were variable. Orange and Tangerine peels contained little or no beta-pinene, both Lemon and Lime peel contained more beta- than alpha-pinene, and the relative amount of beta-myrcene correlated with strong and pleasant aromas (using the human nose detector that I have built-in for cross-validation). This makes good sense since myrcene is one of the most important chemicals used in the perfumery industry.

So as you can probably tell I'm still trying to find some footing for this lab, but I think it's coming along well so far. It's got to be ready to go in January when classes start (week of Jan 5, 2010) which is less than 4 weeks. This project, along with my regularly scheduled research and another educationally-related project (that I haven't even brought up here yet) is keeping me very busy. Oh yeah and I forgot to mention, we're moving to San Diego. But as I said, I'm managing. I have so many wonderful people in my life right now. Thank you all for your support.

Thanksgiving

I am thankful for... family. I married a wonderful man who is a native of So Cal. We are always welcomed by his parents and siblings and enjoy spending time with them very much. We also love our extended family throughout the country, as often as we can see them. They keep us grounded and at the same time encourage us to reach for the stars.
I am thankful for... health. There are always ups and downs with health, but for now we are in a stable period where we aren't consumed with daily worries about our health or the immediate health of anyone we know. Flu season seems to be on everyone's mind, and surely there are many people suffering, but at least some of us were able to get together and celebrate.
I am thankful for... friends. Our friends have shared some of the biggest events of their lives with us, and we in turn do the same for them. Good friends (such as we have) are around in fair weather and when it is a bit dark and stormy. New friends have grown with us through the changes we are making together and independently of each other. We couldn't ask for better!
I am thankful for... stimulation. I am lucky to be surrounded with opportunities for stimulating my mind and body. I can walk into a Science Library, attend a lecture, or participate in a roundtable discussion regarding the latest developments in chemical biology on any given day of the week. I can get together with friends for a bike ride, road run, indoor climb, lap swim, power yoga, push-up contest... you name it and I bet I can make it happen. I feel like I have the world at my fingertips sometimes. And the sheer beauty of the natural world enlivens me.
I am thankful for... community. I am so glad to have a larger network of people to interact with. I feel a sense of belonging to both the university and the city I live in. I enjoy participating in "local" happenings. I feel that ties to people in your community strengthens the quality of life for everyone. I really love going to local events in public lands and seeing the harmony that can exist when people work cooperatively.
I am thankful for... my dear husband. He really is the axis around which I spin. I feel that he has freed me from my own self-doubt. He allows me to flourish in the nourishing environment of unconditional love and support he provides. He is helping me become truer each day. I would be a much lesser person without his help. Thank you, honey!

Gratitude isn't always easy. I've been having a hard time with it lately. Thankfully we have holidays such as Thanksgiving where we can reflect on life in a more positive way. I've been searching for quotes to put on our T-shirts. How about:
"Tough times don't last but tough people do." - A.C. Green

On another note, the holidays mean FOOD at least in my family. I've been biking and running all year long to achieve the level of fitness I have now. Despite my efforts, I learned today that my BMI is still in the "overweight" bracket and I will have to lose another 8 pounds to be in the "average" realm. I have no plans to lose that 8 pounds during the holidays, I would like to make it my resolution for 2010 that I will maintain and perhaps by 2011 reach that sweet goal of 135 pounds and bearly out of the "overweight" category.

Mission Inn Run

Even though I've been commuting to work by bike, I haven't been on many "monster" rides lately. I did half of a big RBC ride to Tom's Farms last weekend but I wasn't able to finish the ride due to a time constraint. For some reason lately I've been more focused on running. Hmmm... psychological damage from the crash? Well at least the flesh wounds have healed. The alternate explaination is that opportunities to enjoy running have presented themselves and so I put a bit more time & money into that hobby for awhile. For one, I got a new pair of shoes....

The unbiased product review website http://www.productwiki.com/ says "The Nike Zoom Nucleus MC+ is a women's trainer shoe retailing at $115. It targets women who train or run long distances. It features a breathable upper with a supportive overlay design and a secure midfoot saddle to help keep users foot in a neutral position. Zoom Air™ unit in heel and forefoot. Decoupled Crash Pad helps reduce overpronation. Supportive shank, BRS 1000™ heel. Duralon™ and Waffle® Fill add cushioning, traction and durability. Nike+ enabled for instant workout feedback when used with a Nike+ SportBand or iPod® nano and Nike + iPod Sport Kit. Wt. 11.0 oz.

I got them because I was feeling some knee pain, and my new running buddy Ronly Schlenk recommended I replace my old shoes if they weren't cut out for long distance runs. I've been running about 5-8 miles per week throughout September and 10-15 miles per week throughout October. I'm not sure what all the stuff in that description means, but I did try on about 8 pairs of shoes in a store called Running Center in Redlands. The staff was very polite, respectful and helpful, carefully observing my stride in a variety of shoes. My old shoes were cheap ($30) and from Big 5 Sporting Goods (I got them on sale). According to MapMyRide, I started using those shoes 2/09/2009 and since then have put 139.86 mi. on them, making their price $0.2145 per mile. Hopefully these new kicks will help me kick butt and last long enough for me to justify the pricetag. And I know that footwear can't be expected to work miracles, but I am hoping this knee pain will be slightly alleviated. It might help if I stretch more regularly.

We (my husband and my mother-in-law and I) discussed during the race today our running goals. Here's what we came up with (1) that we would make team T-shirts with quotes on them (2) to do a 10K as our next event and (3) complete 4 half-marathons in 2010 as a team. If you have T-shirt ideas, if you want one, let us know because we're doing them as Christmas gifts. Talk about living! There's nothing more exhilarating than crossing a finish line with a cheering crowd and super-fast 10K runners sprinting past you, nothing more motivational than watching a sea of runners invading the streets of downtown Riverside for the sheer joy of running together, nothing more fun than running into friends old and new (Matt, Danny, Bernard and Alena, and Hugh) it was great to share this day with you; nothing is a more perfect expression of love than crossing that finish line together!

Road Rash => Rest Day

What can a cyclist do on a rest day? Write a blog post... So I'm on a complete rest day on account of I had my first crash yesterday. And am I feeling some pain today? Yes, I would be lying if I said I wasn't. Is it worse than my monthly menstral cramps? No. I am a bit sad that I won't have foxy arms & legs for halloween, but if it still looks gnarly I can dress up as a crash victim zombie and get some fake blood.

What happened? Well I'm still not sure, crashes happen really fast. What's going through my mind right now is (1) I ran 10 miles the day before and didn't get more than 5 hours sleep two nights in a row and worked long hours all week and I have slow reflexes even on a good day and therefore the bottom line is that I messed up (2) I got new tires and new brakes and this was my first ride after two major mechanical changes to my vehicle so the bike was to blame. The sweethearts I ride with keep offering to take the blame for me going down, but I'm just glad that I didn't take anyone else out. There is no ride worth going on if you injure yourself or someone else. I should have taken it easier that day.

What are my goals? Why put my body through this torture? What am I proving? And to whom? All these are great questions. The truth is that I don't know. I know I'm doing what feels good and right now I am training heavily. I enjoy the views and the outdoors and it brings me a warm and fuzzy feeling that lasts about a day-and-a-half. Which is coincidentially about as long as it takes for the pain from the exertion to fully hit my muscles. About the fourth day without a workout, I start to twitch and feel a pain in my muscles from not doing anything. If I go five or more days, I start to get really crabby at work and at home.

I just read a great article about how exercise can raise the baseline levels of both norepinephrine and dopamine. I think that's what I'm benefiting from. I know that without it I'm suffering. Everyone around me sees it, but I must endure. Luckily the cycling helps.